| Biological Information | |
|---|---|
| Background Information: | The HitHunter cAMP Assay for Small Molecules kits provide a robust, highly sensitive, and easy-to-use assay for monitoring G-protein coupled receptor (GPCR) activation based on 3'-5'-cyclic adenosine monophosphate (cAMP) production in cells. This kit is ideal for screening and profiling small molecules. For testing biologics samples (e.g. crude biologic samples in serum or plasma, purified or non-purified antibodies [anti-ligand or anti-receptor], and hybridoma supernatants), we suggest using the HitHunter cAMP Assay for Biologics which is designed specifically for biologics and large molecule applications. Cell lines from other sources are also compatible with the HitHunter cAMP Assay. The Detailed Assay Protocol sections in this user manual contain procedures for testing agonists on Gαs receptor assays, Gαi Agonist, Gαs and Gαi Antagonist, Anti-Ligand Antibody, as well as suggestions for testing PAMs and NAMs. An alternate protocol suitable for high-throughput screening is also included.
This kit contains all components required to assay 10 96- or 384-well plates. |
| Target Class: | GPCR |
| Target Name: | cAMP |
| Usage | |
| Product Type: | Detection Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Upon arrival, store reagents at –20°C then see user manual for details. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | cAMP Detection |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | HitHunter® |
HitHunter® cAMP Assay for Small Molecules
The HitHunter cAMP Assay for Small Molecules kits is a robust, sensitive, and easy-to-use assay for monitoring GPCR activation based on cAMP production in cells. Ideal for screening & profiling small molecules in testing agonists on Gαs receptor assays, Gαi Agonist, Gαs & Gαi Antagonist, Anti-Ligand Antibody, and testing PAMs and NAMs. Suitable for HTS
User Manuals & Protocols
70-317 HitHunter cAMP Assay for Small Molecules REV4 User Manual
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