Cell Lines and Primary Cells
Engineered cell lines are essential for in vitro cell-based assays replicating biological systems' physiology. Cell lines are developed reflecting the target’s mechanism of action (MOA) with a drug interaction in a standard cell line background. Cell lines can be propagated repeatedly and kept in culture for extended periods of time. These cells provide the ability to deliver more physiologically relevant results in a cost-effective manner reproducibly.
Eurofins DiscoverX® provides the most extensive collection of >1,000 fully qualified, functional cell lines that enable unique drug discovery and development solutions at every stage of your program. Cell lines covering all major drug target classes including GPCRs, cytokines, kinases, checkpoint inhibitors, ion channels, and nuclear proteins such as transcription factors, NHRs, and epigenetic proteins.
With over two decades of experience in generating stable cell lines, Eurofins DiscoverX provides cell lines with unsurpassed quality and industry-validated performance. These cell lines have been used to screen billions of data points to help bring many therapeutics to the market, with many more in the pipeline.
Use the appropriate application-specific detection kits with your cell line model for a complete functional cell-based assay to interrogate the MOA of your small molecule or biologic, measure and rank ligands' potencies, perform binding and functional screens, implement as a lot release assay, and much more.
Product Highlights
- Robust Performance – Rigorously & continuously validated for accurate pharmacology through Eurofins Discovery with billions of data points screened
- Versatile Use – Interrogate diverse MOAs with broad applications ideal for small molecules & biologics screening or profiling
- Diverse – Select relevant cell models from over 50 cell types such as HEK 293, CHO-K1, U2OS, & more
- Established – Implemented in 200+ global programs for potency, stability, & NAb testing
- Stable Cell Lines – Stable cell lines extensively validated with billions of compounds, confirmed stability beyond 10 passages, and usable for a variety of research and drug discovery and development applications
- KILR® Effector Cells – Single donor-derived human primary cytotoxic T cells to measure target cell death using antibody-dependent cell-mediated cytotoxicity and bi-specific antibody-mediated T Cell Redirection assays for screening, characterization, and QC lot release of antibody drugs
- KILR Cell Lines and Pools – Stable cell lines and cell pools to specifically measure target cell death in co-cultures using a simple, non-radioactive, and dye-free method that can be easily implemented in drug discovery through QC lot release programs. Single clonal cell lines include homogeneous expression of a known target protein, while cell pools allow targeting of your protein of interest specific to your immunotherapeutic drug candidate from a variety cell type’s natively expressed cell surface proteins.
- GPCR cAMP and Calcium Cells – Ready-to-assay GPCR cryo-preserved cells validated for cAMP accumulation and calcium flux applications and ideal for screening of agonists, antagonists and various modulators
- Parental Cell Lines – Engineered cells to generate your own assays to measure internalization, β-arrestin recruitment, pharmacotrafficking, and translocation assays of your membrane protein of interest, as well as receptor-specific signaling pathway activation
Eurofins DiscoverX has over 2 decades of experience engineering and developing cell lines with diverse cell backgrounds, expression systems, and reporters.
- A wide selection of diverse cell backgrounds (e.g. HEK 293, CHO, U2OS, 1321N1, Jurkat, Raji, Daudi, and more) in their appropriate adherent or suspension cell models
- Engineered to accurately represent the target biology
- Extensively evaluated target expression using by qPCR, flow cytometry, immunofluorescence, or Western blotting
- Receptor expression and performance stability confirmed for a minimum of 20 passages
- Implement as single cell or co-culture models as relevant to the drug’s MOA
- Vector development using a viral delivery systems based on retrovirus or lentivirus
- Engineered systems using heterologous expression systems with either constitutive (e.g. CMV) or inducible (e.g. tetracycline) promoters
- CRISPR/Cas9 capabilities for knockouts and knock-ins
- Multiple reporter systems for functional readouts including Enzyme Fragment Complementation (EFC) with luminescent read-out
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