Biological Information | |
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Background Information: | The cAMP Hunter™ CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR3, a naturally Gi/Go coupled GPCR. Upon activation of CXCR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | AY242128 |
Target Name: | CXCR3 (IP-10) |
Target Aliases: | Chemokine (C-X-C motif) receptor 3, CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | cAMP Detection |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | cAMP Hunter™ |
cAMP Hunter™ eXpress CXCR3 CHO-K1 GPCR Assay
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The cAMP Hunter™ eXpress CXCR3 CHO-K1 GPCR Assay measures CXCR3 (GPCR) activity via decreased levels of intracellular cAMP. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-234 cAMP Hunter eXpress GPCR Assay REV10 User Manual
View DocumentDatasheets
95-0054E2x Datasheet
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