Biological Information | |
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Background Information: | The cAMP Hunter™ Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Liraglutide) based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This assay has been optimized and qualified with Victoza® (not supplied in the bioassay kit). Victoza® is a registered trademark of Novo Nordisk A/S. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Glucagon Receptor Family |
Sub Family: | Class B |
Accession Number: | NM_002062.3 |
Target Name: | GLP1R |
Target Aliases: | Glucagon-like peptide receptor 1, GLP-1, GLP-1-R, GLP-1R |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | Bioassay Kits |
Application: | Potency; Commercial Release & Stability |
Kit Components: | |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | cAMP Detection |
Detection Method: | Chemiluminescence |
Bioassay Data | |
Qualified With: | Victoza® |
Trademark Statement: | Victoza® is a registered trademark of Novo Nordisk A/S |
Clinical Relevance | |
Therapeutic Area: | Metabolic Diseases |
Additional Information | |
Brand: | cAMP Hunter™ |
cAMP Hunter™ Liraglutide Bioassay Kit
The cAMP Hunter™ Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Liraglutide) based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP.
View bioassay qualification data.
User Manuals & Protocols
70-389 cAMP Hunter Liraglutide Bioassay Kit REV1 User Manual
View DocumentDatasheets
95-0062Y2x Datasheet
View Document