ChemiScreen™ GPR109A Nicotinic Acid Receptor Stable Cell Line

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The ChemiScreen™ GPR109A Nicotinic Acid Receptor Stable Cell Line measures HCAR2 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: HTS201C Size: 2 vials
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Biological Information
Background Information:
The ChemiScreen™ GPR109A Nicotinic Acid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. nicotinic acid) based activation of HCAR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HCAR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HCAR2 , the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Hydroxycarboxylic Acid Receptors
Accession Number:
NM_177551.3
Target Name:
HCAR2 (HCA2 receptor)
Target Aliases:
HCAR2, HCA2/GPR109A/HM74A/NIC1, Hydroxycarboxylic Acids
Target Species:
Human
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS201C Datasheet

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