| Biological Information | |
|---|---|
| Background Information: | The ChemiScreen™ Y4 Neuropeptide Y Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. peptide YY) based activation of NPY4R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NPY4R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of NPY4R , the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. |
| Target Class: | GPCR |
| Family: | Neuropeptide Y Receptors |
| Target Name: | NPY4R |
| Target Aliases: | NPY4R, Y4/PPYR1, Neuropeptide Y |
| Target Species: | Human |
| Usage | |
| Product Type: | Stable Cell Lines |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | Fluorescence |
| Additional Information | |
| Brand: | ChemiSCREEN™ |
ChemiScreen™ Y4 Neuropeptide Y Receptor Stable Cell Line
The ChemiScreen™ Y4 Neuropeptide Y Receptor Stable Cell Line measures NPY4R (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
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HTS204C Datasheet
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