Biological Information | |
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Background Information: | The InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cells included overexpress Bcl2L1 target in an untagged form and Bax binding partner fused to PL. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Family: | BCL2 |
Accession Number: | NM_138578.1 |
Target Name: | Bcl2L1-Bax |
Target Aliases: | BCL2-like 1 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Target Engagement |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Target Engagement |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | InCELL Hunter™ |
InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay
The InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay measures protein binding via compound-induced binding partner disruption and degradation. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-429 InCELL Hunter eXpress Assays User Manual REV0A
View DocumentDatasheets
96-0009E3x Datasheet
View Document