Biological Information | |
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Background Information: | The InCELL Hunter™ U2OS Bcl2-Bim Protein Binding Cell Line provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2-Bim binding via detection of Bim levels. This product uses EFC technology. The cell line included overexpresses Bcl2 target in an untagged form and Bim binding partner fused to PL. When a compound engages the Bcl2 target protein, partner binding is disrupted and the destabilized Bim is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. This stable modified cell line is provided as two vials of cryopreserved cells. |
Family: | BCL2 |
Accession Number: | NM_000633 |
Target Name: | Bcl2-Bim |
Target Aliases: | BCL2 B-cell CLL/lymphoma 2 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Target Engagement |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Target Engagement |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | InCELL Hunter™ |
InCELL Hunter™ U2OS Bcl2-Bim Protein Binding Cell Line
The InCELL Hunter™ U2OS Bcl2-Bim Protein Binding Cell Line measures protein binding via compound-induced binding partner disruption and degradation. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-400 InCELL Hunter Target Engagement Cell Lines REV0 User Manual
View DocumentDatasheets
96-0008C3 Datasheet
View Document