Biological Information | |
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Background Information: | The PathHunter® CHO-K1 C5AR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Complement Peptide Receptor (Anaphylotoxin) |
Sub Family: | Class A |
Accession Number: | NM_001736 |
Target Name: | C5AR1 (C5a1 receptor) |
Target Aliases: | Complement component 5a receptor 1, C5A, C5AR, C5R1, CD88 |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | Bioassay Kits |
Application: | Potency; Commercial Release & Stability |
Kit Components: | |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Degradation |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® CHO-K1 C5AR1 Bioassay Kit
The PathHunter® CHO-K1 C5AR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment
User Manuals & Protocols
70-290 PathHunter GPCR Bioassay Kit REV11 User Manual
View DocumentDatasheets
93-0557Y2x Datasheet
View Document