Biological Information | |
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Background Information: | The PathHunter® CHO-K1 CXCR5 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CXCL13) based activation of CXCR5 GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged CXCR5 and EA-tagged β-Arrestin-2. Activation of CXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Sub Family: | Class A |
Accession Number: | NM_001716 |
Target Name: | CXCR5 (BLR-1) |
Target Aliases: | Burkitt lymphoma receptor, BLR1, CD185, MDR15, CXCR5, CXCR5, Chemokine |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | Bioassay Kits |
Application: | Potency; Commercial Release & Stability |
Kit Components: | |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® CHO-K1 CXCR5 Bioassay Kit
The PathHunter® CHO-K1 CXCR5 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CXCL13) based activation of CXCR5 GPCR activity via detection of β-arrestin recruitment.
User Manuals & Protocols
70-290 PathHunter GPCR Bioassay Kit REV11 User Manual
View DocumentDatasheets
93-0514Y2x Datasheet
View Document