Biological Information | |
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Background Information: | The PathHunter® Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoietin or Epoetin Alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The included cells overexpress EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. This assay has been optimized and qualified with Aranesp® (not supplied in the bioassay kit). Aranesp® is a registered trademark of Amgen Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
Target Class: | Kinase |
Family: | Prolactin Receptor Family |
Accession Number: | NM_000121.3 |
Target Name: | EPOR |
Target Aliases: | EPO-R, Erythropoietin receptor |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Bioassay Kits |
Application: | Potency; Commercial Release & Stability |
Kit Components: | |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Dimerization |
Detection Method: | Chemiluminescence |
Bioassay Data | |
Qualified With: | Aranesp® |
Trademark Statement: | Aranesp® is a registered trademark of Amgen Inc. |
Clinical Relevance | |
Therapeutic Area: | Oncology/Immuno-Oncology |
Additional Information | |
Brand: | PathHunter® |
PathHunter® Epoetin Alfa Bioassay Kit
The PathHunter® Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoietin or Darbepoetin Alfa-induced dimerization of EPOR receptors.
View bioassay qualification data.
User Manuals & Protocols
70-315 PathHunter Epoetin Alfa Bioassay Kit REV3 User Manual
View DocumentDatasheets
93-0965Y3x Datasheet
View Document