| Biological Information | |
|---|---|
| Background Information: | The PathHunter® ADRA1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged ADRA1B and EA-tagged β-Arrestin-2. Activation of ADRA1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Adrenoceptor |
| Sub Family: | Class A |
| Coupling: | Gq |
| Accession Number: | NM_000679 |
| Target Name: | ADRA1B (α1B-adrenoceptor) |
| Target Aliases: | Adrenergic receptor alpha 1B, ADRA1, ALPHA1BAR, ADRA1B, α1B |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| β-Arrestin isoform: | β-Arrestin-2 |
| Assay Measures: | β-Arrestin Recruitment |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress ADRA1B CHO-K1 β-Arrestin GPCR Assay
The PathHunter® eXpress ADRA1B CHO-K1 β-Arrestin GPCR Assay measures ADRA1B (GPCR) activity via recruitment of β-Arrestin 2. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-255 PathHunter B Arrestin eXpress GPCR Assay REV4 User Manual
View DocumentDatasheets
93-0274E2x Datasheet
View Document