PathHunter® eXpress CDC25A Degradation Assay

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The PathHunter® eXpress CDC25A Degradation Assay measures pathway activation-induced degradation of CDC25A target protein. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0765E3CP0L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The PathHunter® eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cells included overexpress CDC25a target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Phosphatase
Family:
Dual-Specific Phosphatase
Sub Family:
Protein Tyrosine
Accession Number:
NM_001789.2
Target Name:
CDC25A
Target Aliases:
Cell division cycle 25 homolog A
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Degradation
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-278 PathHunter eXpress HEK-293 IkB Degradation Assay REV1 User Manual

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Datasheets

93-0765E3x Datasheet

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