Biological Information | |
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Background Information: | The PathHunter® eXpress HEK 293 IκB Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the NFκB pathway. This kit enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cells included overexpress IκB target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | Signaling Pathway |
Accession Number: | NM_020529 |
Target Name: | IκB |
Target Aliases: | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
Target Species: | Human |
Cell Background: | HEK293 |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Degradation |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® eXpress HEK 293 IkappaB Degradation Assay
The PathHunter® eXpress HEK 293 IκB Degradation Assay measures pathway activation-induced degradation of IκB target protein. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-278 PathHunter eXpress HEK-293 IkB Degradation Assay REV1 User Manual
View DocumentDatasheets
93-0538E1x Datasheet
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