| Biological Information | |
|---|---|
| Background Information: | The PathHunter® P2RY2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. UTP, ATP) based internalization of P2RY2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | P2Y Receptors |
| Sub Family: | Class A |
| Coupling: | Gq |
| Accession Number: | AY136753 |
| Target Name: | P2RY2 |
| Target Aliases: | Purinergic receptor P2Y, G-protein coupled, 2, ATP receptor, HP2U, P2RU1, P2U, P2U nucleotide receptor, P2U purinoceptor 1, P2U1, P2UR, P2Y purinoceptor 2, P2Y2, P2Y2R, purinergic receptor P2Y, G-protein coupled, 2, purinergic receptor P2Y2, purinoceptor P2Y2 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Internalization |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress P2RY2 Activated GPCR Internalization Assay
The PathHunter® eXpress P2RY2 Activated GPCR Internalization Assay measures P2RY2 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-261 PathHunter eXpress Activated GPCR Internalization Assays REV7 User Manual
View DocumentDatasheets
93-0719E3
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