| Biological Information | |
|---|---|
| Background Information: | The PathHunter® PRLR-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses PRLR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged PRLR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | Cytokine Receptor |
| Family: | Prolactin Receptor Family |
| Accession Number: | NM_000949 |
| Target Name: | PRLR-JAK1 |
| Target Aliases: | Prolactin receptor / Janus kinase 1, JTK3, AIIDE, JAK1A, JAK1B |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Functional Activity |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress PRLR-JAK1 Functional Assay
The PathHunter® eXpress PRLR-JAK1 Functional Assay measures PRLR cytokine receptor activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.
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