PathHunter® eXpress RXFP4 CHO-K1 β-Arrestin GPCR Assay

Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.

The PathHunter® eXpress RXFP4 CHO-K1 β-Arrestin GPCR Assay measures RXFP4 (GPCR) activity via recruitment of β-Arrestin 2. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0701E2CP0L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The PathHunter® RXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged RXFP4 and EA-tagged β-Arrestin-2. Activation of RXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Relaxin Family Peptide Receptors
Sub Family:
Class A
Coupling:
Gi/Go
Accession Number:
NM_181885.2
Target Name:
RXFP4
Target Aliases:
Relaxin family peptide receptor 4, G protein-coupled receptor 100, G-protein coupled receptor 100, G-protein coupled receptor GPCR142, GPCR142, GPR100, RLN3 receptor 2, RLN3R2, RXFPR4, insulin-like peptide INSL5 receptor, relaxin 3 receptor 2, relaxin family peptide receptor 4, relaxin-3 receptor 2, relaxin/insulin-like family peptide receptor 4
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-255 PathHunter B Arrestin eXpress GPCR Assay REV4 User Manual

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Datasheets

93-0701E2_PathHunter_eXpress RXFP4 CHO-K1 beta-Arrestin GPCR Assay

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