Biological Information | |
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Background Information: | The PathHunter® RXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged RXFP4 and EA-tagged β-Arrestin-2. Activation of RXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Relaxin Family Peptide Receptors |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | NM_181885.2 |
Target Name: | RXFP4 |
Target Aliases: | Relaxin family peptide receptor 4, G protein-coupled receptor 100, G-protein coupled receptor 100, G-protein coupled receptor GPCR142, GPCR142, GPR100, RLN3 receptor 2, RLN3R2, RXFPR4, insulin-like peptide INSL5 receptor, relaxin 3 receptor 2, relaxin family peptide receptor 4, relaxin-3 receptor 2, relaxin/insulin-like family peptide receptor 4 |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® eXpress RXFP4 CHO-K1 β-Arrestin GPCR Assay
User Manuals & Protocols
70-255 PathHunter B Arrestin eXpress GPCR Assay REV4 User Manual
View DocumentDatasheets
93-0701E2_PathHunter_eXpress RXFP4 CHO-K1 beta-Arrestin GPCR Assay
View Document