Biological Information | |
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Background Information: | The PathHunter® eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | Nuclear Protein |
Accession Number: | NM_015321.1 |
Target Name: | TORC1 (CRTC1) |
Target Aliases: | CREB regulated transcription coactivator 1 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Translocation |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® eXpress TORC1 Nuclear Translocation Assay
The PathHunter® eXpress TORC1 Nuclear Translocation Assay measures CRTC/CREB pathway activation via nuclear translocation of TORC1. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-300 PathHunter eXpress Nuclear Translocation Assays REV6 User Manual
View DocumentDatasheets
93-0833E3x Datasheet
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