Biological Information | |
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Background Information: | The PathHunter® GLP1R Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. exendin-4) based internalization of GLP1R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged GLP1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GLP1R receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Glucagon Receptor Family |
Sub Family: | Class B |
Coupling: | Gs |
Accession Number: | NM_002062.3 |
Target Name: | GLP1R |
Target Aliases: | Glucagon-like peptide receptor 1, GLP-1, GLP-1-R, GLP-1R |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Internalization |
Detection Method: | Chemiluminescence |
Clinical Relevance | |
Therapeutic Area: | Metabolic Diseases |
Additional Information | |
Brand: | PathHunter® |
PathHunter® GLP1R Total GPCR Internalization U2OS Cell Line
The PathHunter® GLP1R Total GPCR Internalization U2OS Cell Line measures GLP1R (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-260 PathHunter Total GPCR Internalization Assay REV6 User Manual
View DocumentDatasheets
93-1075C3 Datasheet
View Document