Biological Information | |
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Background Information: | The PathHunter® ICOS Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. ICOS-L) based activation of ICOS receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ICOS target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Checkpoint Receptor |
Family: | IgSF (immune co-stimulatory Receptor) |
Accession Number: | NM_012092.3 |
Target Name: | ICOS |
Target Aliases: | Inducible T-cell costimulator |
Target Species: | Human |
Cell Background: | Jurkat |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Signaling |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® Jurkat ICOS Signaling Assay
The PathHunter® Jurkat ICOS Signaling Assay measures ICOS signal by recruitment of SH2 domain protein to the activated receptor. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-394 PathHunter Checkpoint Signaling Assay-IgSF_REV3
View DocumentDatasheets
93-1127C19
View Document