Biological Information | |
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Background Information: | The PathHunter® MC2R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC2R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MC2R and EA-tagged β-Arrestin 2. Activation of MC4R stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Melanocortin |
Coupling: | Gs |
Accession Number: | NM_005912.2 |
Target Name: | MC2R |
Target Aliases: | ACTH receptor, ACTH-R, Adrenocorticotropin receptor, Melanocortin receptor 2 (MC2-R), Adrenocorticotropic hormone receptor |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | ?-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® MC2R β-Arrestin Cell Line (U2OS)
The PathHunter® MC2R β-Arrestin Cell Line (U2OS) measures MC2R (GPCR) activity via recruitment of β-Arrestin 2. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-247-PathHunter-Beta-Arrestin-Assay-for-GPCR-Cell-Lines_REV5-1 User Manual
View DocumentDatasheets
93-1189C3 Datasheet
View Document