PathHunter® MTNR1B Total GPCR Internalization U2OS Cell Line

The PathHunter® MTNR1B Total GPCR Internalization U2OS Cell Line measures MTNR1B (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0881C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® MTNR1B Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. melatonin) based internalization of MTNR1B via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged MTNR1B and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MTNR1B receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Melanocortin Receptors
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_005959.3
Target Name:	MTNR1B
Target Aliases:	Melatonin receptor 1B, FGQTL2, MEL-1B-R, MT2
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®