PathHunter® SREBP1c Nuclear Translocation Cell Line (U2OS)

The PathHunter® SREBP1c Nuclear Translocation Cell Line (U2OS) supports measurement of attenuated transactivation of SREBP1c cognate genes that contribute hyperlipidemia via nuclear translocation of SREBP1c. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1175C3 Size: 2 vials

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Biological Information
Background Information:	PathHunter® Nuclear Translocation cell lines are engineered to co-express two proteins: a) Enzyme Donor (ED) tagged target protein; b) an Enzyme Acceptor (EA), which is localized to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into the nucleus, which will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme, that will hydrolyze substrate and generate a chemiluminescent signal; or b) induce the ED-tagged protein to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal. Some nuclear translocation assays will also co-express an untagged secondary protein involved in the pathway of interest.
Target Class:	Nuclear Protein
Accession Number:	NP_001308025.1
Target Name:	SREBP1c
Target Aliases:	HMD, IFAP2, SREBF1, bHLHd1
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Translocation
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Translocation

Clinical Relevance
Therapeutic Area:	Metabolic Diseases

Additional Information
Brand:	PathHunter®