Biological Information | |
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Background Information: | The PathHunter® U2OS CFTR-ΔF508 MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting CFTR. This cell line enables assessment of chaperone-based stabilization of CFTR-ΔF508 via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant CFTR-ΔF508, which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant CFTR, it is trafficked to the membrane, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Ion Channel |
Family: | Transporter |
Sub Family: | Other |
Accession Number: | NP_000483.3 |
Target Name: | CFTR-ΔF508 |
Target Aliases: | Cystic fibrosis transmembrane conductance regulator |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Pharmacotrafficking |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Pharmacotrafficking |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS CFTR-deltaF508 MEM-EA Pharmacotrafficking Assay
The PathHunter® U2OS CFTR-ΔF508 MEM-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant CFTR GPCR via detection of localization to the membrane. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-302 PathHunter Pharmacotrafficking Assays REV4 User Manual
View DocumentDatasheets
93-0987C3 Datasheet
View Document