Biological Information | |
---|---|
Background Information: | The PathHunter® U2OS ErbB2-ErbB3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated ErbB2 and ErbB3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ErbB2 and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, ErbB3 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Kinase |
Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
Sub Family: | Protein Tyrosine |
Accession Number: | NM_004448.2 |
Target Name: | ErbB2-ErbB3 |
Target Aliases: | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2) |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Functional Activity |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS ErbB2-ErbB3 Functional Assay
The PathHunter® U2OS ErbB2-ErbB3 Functional Assay measures ErbB2-ErbB3 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-269 PathHunter Receptor Tyrosine Kinase Functional Assay REV4 User Manual
View DocumentDatasheets
DRx_DS_93-0535C3_PHCb-RTK_U2OS_ErbB2-ErbB3_20231013
View Document