Biological Information | |
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Background Information: | The PathHunter® U2OS FOXO3 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the PI3 pathway. This cell line enables assessment of ligand (e.g. wortmannin) based pathway activation via detection of resulting FOXO3 nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged FOXO3 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of FOXO3 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Nuclear Protein |
Family: | Forkhead Transcription Factor |
Accession Number: | NM_201559.2 |
Target Name: | FOXO3 |
Target Aliases: | forkhead box O3, FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Translocation |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS FOXO3 Nuclear Translocation Cell Line
The PathHunter® U2OS FOXO3 Nuclear Translocation Cell Line measures PI3 pathway activation via nuclear translocation of FOXO3. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-263 PathHunter NHR Assays REV6 User Manual
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93-0876C3 Datasheet
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