Biological Information | |
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Background Information: | The PathHunter® IL12RB1/IL12RB2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. IL-12) induced dimerization of IL12RB1/IL12RB2 receptors. This product uses EFC technology. The cell line included overexpresses IL12RB2 target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL12RB1/IL12RB2 , detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Cytokine Receptor |
Family: | IL-12 Receptor Family |
Accession Number: | NM_005535 |
Target Name: | IL12RB1/IL12RB2 |
Target Aliases: | IL-12R, IL12R, IL-12 Receptor, Interleukin 12 receptor, beta 1, CD212, IL-12R-BETA1, IL12RB, IMD30 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Dimerization |
Detection Method: | Chemiluminescence |
Clinical Relevance | |
Therapeutic Area: | Inflammation/Allergy |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS IL12RB1/IL12RB2 Dimerization Cell Line
The PathHunter® U2OS IL12RB1/IL12RB2 Dimerization Cell Line measures ligand-induced dimerization of IL12RB1/IL12RB2 . This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-303 PathHunter Dimerization Assay REV3 User Manual
View DocumentDatasheets
93-1041C3 Datasheet
View Document