PathHunter® U2OS INSRb Internalization Assay

The PathHunter® U2OS INSRb Internalization Assay measures INSRb receptor tyrosine kinase activity via activation-induced receptor internalization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0808C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® INSRb RTK Internalization Assay is a stable, engineered cell line for use in studying molecules targeting RTKs. This cell line enables assessment of RTK activation via detection of INSRb internalization to endosomes. This product uses EFC technology. The included cell line overexpresses PK-tagged INSRb and EA-tagged endosome fusion proteins. Upon ligand-induced activation of the receptor, internalization of the receptor to the endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Kinase
Family:	Receptor Typosine Kinase (RTK): Growth Factor Receptor
Sub Family:	Protein Tyrosine
Accession Number:	NM_000208
Target Name:	INSRb
Target Aliases:	Insulin receptor, isoform B, HHF5, CD220
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®