Biological Information | |
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Background Information: | The PathHunter® U2OS LCK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting CTKs. This cell line enables assessment of CTK activation via detection of resulting GHR phosphorylation-induced SH2 domain protein recruitment, mediated by LCK-JAK2 fusion protein. This product uses EFC technology. The included cell line overexpresses PK-tagged relevant cytokine receptor and EA-tagged SH2 domain protein alongside an engineered fusion of the cytokine receptor binding domain of JAK2 (Janus Kinase 2) to the catalytic domain from the CTK of interest, LCK. Upon activation of the cytokine receptor, the CRBD-CTK fusion phosphorylates the receptor, leading to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Kinase |
Family: | Cytosolic Tyrosine Kinase |
Sub Family: | Protein Tyrosine |
Accession Number: | NM_000163 |
Target Name: | Lck |
Target Aliases: | Growth hormone receptor |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Functional Activity |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS LCK Activity Assay
The PathHunter® U2OS LCK Activity Assay measures LCK CTK activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-249 PathHunter Cytosolic Tyrosine Kinase Activity Assay REV4 User Manual
View DocumentDatasheets
93-1100C3 Datasheet
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