Biological Information | |
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Background Information: | The PathHunter® U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting mRHO. This cell line enables assessment of chaperone-based stabilization of mRHO(P23H) via detection of rescued membrane-endosome trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant mRHO(P23H), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant mRHO, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Opsin Receptors |
Coupling: | Gt |
Accession Number: | NM_145383.1 |
Target Name: | RHO(P23H) |
Target Aliases: | Rhodopsin |
Target Species: | Mouse |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Pharmacotrafficking |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Pharmacotrafficking |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay
The PathHunter® U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant mRHO GPCR via detection of localization to the endosome. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-302 PathHunter Pharmacotrafficking Assays REV4 User Manual
View DocumentDatasheets
93-0990C3 Datasheet
View Document