Biological Information | |
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Background Information: | The PathHunter® U2OS PDGFRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PDGFRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Kinase |
Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
Sub Family: | Protein Tyrosine |
Accession Number: | NM_006206.4 |
Target Name: | PDGFRa |
Target Aliases: | PDGFRA, Platelet-derived growth factor receptor alpha, CD140A, PDGFR2, PDGFR-2 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Functional Activity |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS PDGFRa Functional Assay
The PathHunter® U2OS PDGFRa Functional Assay measures PDGFRa receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-269 PathHunter Receptor Tyrosine Kinase Functional Assay REV4 User Manual
View DocumentDatasheets
DRx_DS_93-0823C3_PHCb-RTK_U2OS_PDGFRalpha_20231013
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