Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells

The Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells measure CHRM5 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

Catalog #: HTS075RTA Size: 2 vials

Biological Information
Background Information:	The Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM5 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CHRM5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM5 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:	GPCR
Family:	Acetylcholine Receptor (Muscarinic)
Accession Number:	NM_012125
Target Name:	CHRM5
Target Aliases:	Cholinergic receptor, muscarinic 5, HM5, CHRM5, M5, Acetylcholine (Muscarinic)
Target Species:	Human

Usage
Product Type:	Frozen Cells
Application:	Drug Discovery & Development
Storage Conditions:	Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Measures:	Calcium Flux
Detection Method:	Fluorescence

Additional Information
Brand:	ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS075RTA Datasheet

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