Biological Information | |
---|---|
Background Information: | The Ready-to-Assay™ MC5 Melanocortin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MC5R alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MC5R , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
Target Class: | GPCR |
Family: | Melanocortin Receptors |
Accession Number: | NM_005913 |
Target Name: | MC5R |
Target Aliases: | Melanocortin receptor 5, MC2, MC5R, MC5, Melanocortin |
Target Species: | Human |
Usage | |
Product Type: | Frozen Cells |
Application: | Drug Discovery & Development |
Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiSCREEN™ |
Ready-to-Assay™ MC5 Melanocortin Receptor Frozen Cells
![Awaiting product image](https://www.discoverx.com/content/uploads/2023/08/edx-default.jpg)
The Ready-to-Assay™ MC5 Melanocortin Receptor Frozen Cells measure MC5R (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
View DocumentDatasheets
HTS155RTA Datasheet
View Document