| Biological Information | |
|---|---|
| Background Information: | The Ready-to-Assay™ OT Oxytocin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. oxytocin) based activation of OXTR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress OXTR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of OXTR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
| Target Class: | GPCR |
| Family: | Vasopressin & Oxytocin Receptors |
| Accession Number: | NM_000916 |
| Target Name: | OXTR |
| Target Aliases: | OXTR, OT, Vasopressin & Oxytocin |
| Target Species: | Human |
| Usage | |
| Product Type: | Frozen Cells |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | Fluorescence |
| Additional Information | |
| Brand: | ChemiSCREEN™ |
Ready-to-Assay™ OT Oxytocin Receptor Frozen Cells
The Ready-to-Assay™ OT Oxytocin Receptor Frozen Cells measure OXTR (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
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