| Biological Information | |
|---|---|
| Background Information: | SPRINTer™ Protein Turnover Biosensor cell lines are engineered to introduce the EFC Enzyme Donor (ED) into the endogenous locus of the desired target gene. Expression of the target gene from its native promoter results in production of an ED-tagged target protein. Treatment of the engineered Biosensor cells with therapeutics that promote turnover of the target protein produces a decrease in EFC signal. Addition of exogenous Enzyme Acceptor (EA) and buffer lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal, allowing quantitation of drug-induced changes in ED-target protein levels. |
| Target Class: | Epigenetics |
| Family: | Transcription factor |
| Accession Number: | NM_001369512 |
| Target Name: | STAT3 |
| Target Aliases: | "signal transducer and activator of transcription 3, STAT3, APRF HIES ADMIO ADMIO1" |
| Target Species: | Human |
| Cell Background: | A549 |
| Usage | |
| Product Type: | Stable Cell Lines |
| Application: | Targeted Protein Degradation |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Targeted Protein Degradation (TPD) |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | SPRINTer™ |
SPRINTer™ STAT3 Protein Turnover Biosensor Cell Line (A549)
The SPRINTer™ STAT3 (A549) Protein Turnover Biosensor Cell Line is a stable clonal cell line used for monitoring drug-induced protein turnover of STAT3.
User Manuals & Protocols
70-425_SPRINTer_Endogenous_Protein_Turnover_Biosensor_Cell_Line User Manual
View DocumentDatasheets
91-1011C15 Datasheet
View Document