Products / Assay / 93-0572Y2-00133
Contact a Specialist

PathHunter® CHO-K1 GLP2R Bioassay Kit

The PathHunter CHO-K1 GLP2R Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Recombinant Human Complement C5a) based on activation of GLP2R GPCR activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0572Y2-00134 1000 dp (10 x 96-well) $8,975.00
93-0572Y2-00133 200 dp (2 x 96-well) $2,780.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

Related Family Members:

Target/Description
Glucagon-like peptide receptor 2
Group
Glucagon
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
NM_004246.1
Agonist/Ligand
Glucagon-like Peptide 2 (GLP-2)
Assay Measures
β-Arrestin
Bioassay Target Name
GLP2R
Assay Qualified With
GLP-2
The following graph is an example of a typical dose-response curve for the GLP2R Bioassay generated using the protocol outlined in this user manual. The data shows potent, dose-dependent recruitment of β-arrestin, when treated with GLP-2.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gs