PathHunter® eXpress Flt3 Activity Assay

PathHunter® RTK Activity cell lines are engineered to express a fusion of the intracellular catalytic domain from the target RTK and the extracellular ligand binding domain of EGFR. This fusion protein is tagged with ProLink™ (PK). The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain. Ligand induced cross-phosphorylation of the PK-tagged fusion protein leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0901E3CP16S 100 dp (1 x 96-well) $859.00
93-0901E3CP16M 200 dp (2 x 96-well) $1249.00
93-0901E3CP16L 1000 dp (10 x 96-well) $4939.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Epidermal growth factor receptor
Group
Receptor Tyrosine Kinase
SH2 Domain
PLCG2
Cell Background
U2OS
Species
Human
Accession Number
NM_005228.3
Agonist/Ligand
Recombinant Human EGF
Antagonist/Inhibitor
PKC-412
Assay Measures
Receptor Phosphorylation
Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then pre-incubated with inhibitor, followed by stimulation with the control agonist . Following agonist stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A