PathHunter® Growth Hormone Bioassay Kit

The PathHunter Growth Hormone Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Glucagon-like Peptide 2 (GLP-2)) based on activation of GHR Kinase activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged GHR and EA-tagged . Activation of GHR stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with hGH (not supplied in the bioassay kit). hGH is a registered trademark of . Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0756Y3-00024 1000 dp (10 x 96-well) $8,259.00
93-0756Y3-00023 200 dp (2 x 96-well) $2,399.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Growth hormone receptor
Group
Cytosolic Tyrosine Kinase
SH2 Domain
PLCG1
Cell Background
U2OS
Receptor Species
Human
Agonist/Ligand
Recombinant Human Growth Hormone
Assay Measures
SH2 Recruitment
Bioassay Target Name
GHR-JAK1
Assay Qualified With
hGH
The above graph is representative dose-response curve with hGH. Using the protocol outlined in the user manual, the data show a potent, dose-dependent phosphorylation of the receptor by the cytosolic tyrosine kinase JAK1 in U2OS cells when treated with a dose-response of hGH.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® Human Growth Hormone Bioassay Kit

    PathHunter® Human Growth Hormone (hGH) Bioassay kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study hGH potency and neutralizing antibodies. The Bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, dilution buffer, and...

    [User Manual] PathHunter® Human Growth Hormone Bioassay Kit