PathHunter® eXpress IL17RD/TNFR2 Dimerization Assay

The PathHunter® Dimerization assay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-1068E3CP5S 100 dp (1 x 96-well) $1389.00
93-1068E3CP5M 200 dp (2 x 96-well) $2069.00
93-1068E3CP5L 1000 dp (10 x 96-well) $7159.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Interleukin-17 receptor D precursor
Group
Interleukin Receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_017563
Agonist/Ligand
TNFα (human)
Assay Measures
IL17RD/TNFR2 Dimerization
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® eXpress Dimerization Assay Kit

    PathHunter® eXpress Dimerization Assay Kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study receptor-receptor interactions at the cell surface. The eXpress kits contain all the reagents needed for a complete assay  [User Manual] PathHunter® eXpress Dimerization Assay Kit