PathHunter® Jurkat SIRPa Signaling Bioassay Kit

The PathHunter Jurkat SIRPa Signaling Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. TGR5 Receptor Agonist) based on activation of SIRPa Checkpoint activity via detection of cAMP. The included cell line overexpresses ProLink-tagged SIRPa and EA-tagged . Activation of SIRPa stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-1135Y19-00130 1000 dp (10 x 96-well) $8,969.00
93-1135Y19-00129 200 dp (2 x 96-well) $2,419.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Signal regulatory protein alpha
Group
Signal regulatory protein
SH2 Domain
SHP1
Cell Background
Jurkat
Receptor Species
Human
Accession Number
NM_001040022.1
Agonist/Ligand
Anti-CD47 Antibody (Ab-2; Clone B6H12.2)
Assay Measures
SH2 Recruitment
Bioassay Target Name
SIRPa
Assay Qualified With
Anti-CD47 Antibody
The following graph is an example of a typical dose-response curve for the SIRPα signaling bioassay generated using the protocol outlined in this manual. The data shows a potent, dose-dependent inhibition of the CD47-SIRPα signaling axis, when treated with the control anti-CD47 antibody. The plate was read on the EnVision® Multimode Plate Reader and data analysis done using GraphPad Prism.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® Jurkat SIRPα Signaling Bioassay Kit

    The PathHunter SIRPα Signaling Bioassay Kit provides a simple, highly specific assay to enable discovery and development of small molecule and biologic drugs targeting the CD47-SIRPα signaling axis. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control antibody, and assay plates. The cryop...

    [User Manual] PathHunter® Jurkat SIRPα Signaling Bioassay Kit