PathHunter® Jurkat SIRPa Signaling Bioassay Kit

The PathHunter SIRPa Signaling Bioassay consists of human cells engineered to stably express an ED-tagged immune checkpoint receptor, SIRPa, while EA is fused to either the full length or the phosphotyrosine-binding SH2 domain of an intracellular signaling protein, SHP1. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates.

Catalog No. Size Price Quantity
93-1135Y19-00130 1000 dp (10 x 96-well) $8,969.00
93-1135Y19-00129 200 dp (2 x 96-well) $2,419.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Signal regulatory protein alpha
Group
Signal regulatory protein
SH2 Domain
SHP1
Cell Background
Jurkat
Species
Human
Accession Number
NM_001040022.1
Agonist/Ligand
Anti-CD47 Antibody (Ab-2; Clone B6H12.2)
Assay Measures
SH2 Recruitment
Bioassay Target Name
SIRPa
Assay Qualified With
Anti-CD47 Antibody
The following graph is an example of a typical dose-response curve for the SIRPα signaling bioassay generated using the protocol outlined in this manual. The data shows a potent, dose-dependent inhibition of the CD47-SIRPα signaling axis, when treated with the control anti-CD47 antibody. The plate was read on the EnVision® Multimode Plate Reader and data analysis done using GraphPad Prism.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® Jurkat SIRPα Signaling Bioassay Kit

    The PathHunter SIRPα Signaling Bioassay Kit provides a simple, highly specific assay to enable discovery and development of small molecule and biologic drugs targeting the CD47-SIRPα signaling axis. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control antibody, and assay plates. The cryop...

    [User Manual] PathHunter® Jurkat SIRPα Signaling Bioassay Kit