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PathHunter® Panitumumab Bioassay Kit

The PathHunter Panitumumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. FGF-19) based on activation of EGFR/ErbB2 Kinase activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged EGFR/ErbB2 and EA-tagged . Activation of EGFR/ErbB2 stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with ‎Vectibix® (not supplied in the bioassay kit). ‎Vectibix® is a registered trademark of Amgen Inc.. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-1051Y3-00094 1000 dp (10 x 96-well) $9,980.00
93-1051Y3-00093 200 dp (2 x 96-well) $2,979.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Epidermal growth factor receptor
Group
Receptor Tyrosine Kinase
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_005228.3
Agonist/Ligand
Recombinant Human EGF
Antagonist/Inhibitor
Vectibix (Panitumumab)
Assay Measures
EGFR/ErbB2 Dimerization
Bioassay Target Name
EGFR/ErbB2
Assay Qualified With
‎Vectibix®
Drug Trademark Owner
‎Vectibix® is a registered trademark of Amgen Inc.
The above graph is an example of a typical dose-response curve for the Panitumumab Bioassay, generated using the protocol outlined in the user manual. Data was acquired using the EnVision Microplate Reader (PerkinElmer).
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A

  • [User Manual] PathHunter® Panitumumab Bioassay Kit

    PathHunter Panitumumab Bioassay kits provide a functional, robust, highly sensitive, and easy-to-use cell-based assay to study panitumumab potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The pre-valid...

    [User Manual] PathHunter® Panitumumab Bioassay Kit