The PathHunter Sargramostim Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Anti-PD1 Antibody [NAT105]) based on activation of CSF2RB/CSF2RA Cytokine activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged CSF2RB/CSF2RA and EA-tagged . Activation of CSF2RB/CSF2RA stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Leukine® (not supplied in the bioassay kit). Leukine® is a registered trademark of Genzyme Corporation (licensed). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.