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PathHunter® Tocilizumab Bioassay Kit

The PathHunter Tocilizumab Bioassay is an application of the DiscoverX Dimerization Assay platform. The assay is designed to detect the ligand-induced interaction of the interleukin-6 receptor (IL-6R) with the interleukin-6 signal transducer protein (IL-6ST). The cells have been engineered to co-express IL-6ST fused to PK, and IL-6R fused to EA. Binding of an agonist to the IL-6R causes multimerization of the IL-6R and IL-6ST receptors, resulting in activation of downstream signaling events. This brings the two b-gal fragments into close proximity, forcing complementation. Heterodimerization of the two receptor chains results in the formation of a functional b-gal enzyme that hydrolyzes the substrate to generate a chemiluminescent signal. Blocking IL-6 binding with an anti-ligand or anti-receptor antibody can prevent this interaction, resulting in a loss of signal. The bioassay kit contains all reagents needed for the assay, including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates.

Catalog No. Size Price Quantity
93-1045B3-00110 1000 dp (10 x 96-well) $8251.00
93-1045B3-00109 200 dp (2 x 96-well) $2352.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
interleukin 6 receptor
Group
Interleukin Receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_000565.3
Agonist/Ligand
IL-6
Assay Measures
Dimerization
Bioassay Target Name
IL6R/IL6ST
Assay Qualified With
Actemra
Drug Trademark Owner
Actemra is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A