Products / Assay / 93-0985Y3-00135
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PathHunter® U2OS IL10RA/IL10RB Dimerization Bioassay Kit

The PathHunter U2OS IL10RA/IL10RB Dimerization Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Erythropoietin) based on activation of IL10RA/IL10RB Cytokine activity via detection of Dimerization. The included cell line overexpresses ProLink-tagged IL10RA/IL10RB and EA-tagged . Activation of IL10RA/IL10RB stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0985Y3-00136 1000 dp (10 x 96-well) $8,975.00
93-0985Y3-00135 200 dp (2 x 96-well) $2,780.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Interleukin 10 receptor, alpha
Group
Interleukin Receptor
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_001558.3
Agonist/Ligand
IL-10
Assay Measures
Dimerization
Bioassay Target Name
IL10RA/IL10RB
Assay Qualified With
IL-10
The graph is an example of a typical dose-response curve for the IL-10RA/IL-10RB Bioassay generated using the protocol outlined in the user manual. The data shows potent, dose-dependent heterodimerization of the two receptors, when treated with IL-10.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A