PathHunter® U2OS TrkA-p75 Bioassay Kit

The PathHunter TrkA-p75 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to study drug potency and neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to QC lot release. This bioassay has been optimized for a 96-well format. The protocol can be optimized further for running the assay in a high-throughput 384-well format.

Catalog No. Size Price Quantity
93-0529Y3-00148 1000 dp (10 x 96-well) $7729.00
93-0529Y3-00147 200 dp (2 x 96-well) $2249.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Neurotrophic tyrosine kinase receptor type 1
Group
Receptor Tyrosine Kinase
SH2 Domain
SHC1
Cell Background
U2OS
Species
Human
Accession Number
NM_002529
Agonist/Ligand
Recombinant Human b-NGF
Assay Measures
SH2 Recruitment
Bioassay Target Name
TrkA-p75
The following graph is an example of a typical dose response curve for the TrkA-p75 Bioassay generated using the protocol outlined in this manual. The data shows potent, dose-dependent SH2 recruitment, when TrkA-p75 is treated with Recombinant Human β-NGF.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® TrkA-p75 Bioassay Kit

    The PathHunter TrkA-p75 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to study drug potency and neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The cryopreserved cells have been manufactured to en...

    [User Manual] PathHunter® TrkA-p75 Bioassay Kit