PathHunter® CHO-K1 GPR139 β-Arrestin Orphan GPCR Cell Line

PathHunter® β-Arrestin Orphan GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0412C2A 2 vials $19,700.00

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Related Family Members:

Receptor Family
Class A Orphan
Accession Number
Receptor Species
β-Arrestin isoform
Prolink™ Tag
Cell Background
Figure 1. Cell lysates prepared from PathHunter® β-Arrestin Orphan GPCR cell lines were treated with PNGase F (Glyko; Cat. #GKE-5003), run on a SDS-PAGE gel and analyzed using the PathHunter® EAstern Blot Kit (DRx: 93-0053). Untreated lane resolves a band of appropriate size corresponding to GPCR-PK fusion protein and the PNGase F treated lane resolves a deglycosylated band indicative of proper expression and folding of GPCR protein.
Figure 2. PathHunter® β-Arrestin Orphan GPCR cells were analyzed for basal activity as well as GPCR-ProLinkTM expression by comparing the ratio of signal between untreated cells and cells treated with saturating amounts of exogenous EA, using ProLink™ Detection Kit (DRx: 92-0006). Signal from complementation of ProLink™ and EA fragments correlates to the amount of GPCR-PK expression in the cell line.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,649.00
92-3107G AssayComplete™ Cell Culture Kit-107 Kit $359.00
92-5102FR AssayComplete™ Freezing Reagent F2 2 x 50 mL $299.00
93-0563R1A AssayComplete™ Cell Plating 1 Reagent 100 mL $299.00
92-4102TR AssayComplete™ Thawing Reagent T2 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $189.00
92-0030 AssayComplete™ G418 20 mL $189.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00