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PathHunter® HEK 293 IGF1R Functional Assay

PathHunter® RTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged RTK, an Enzyme Acceptor (EA) tagged SH2 domain, and, in some cases, an untagged co-receptor. Activation of the RTK-PK induces receptor dimerization, leading to SH2-EA recruitment, and forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0505C1 2 vials $20595.00

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Related Family Members:

Receptor Family
Receptor Tyrosine Kinase
Coupling
N/A
Accession Number
NM_000875
Species
Human
Prolink™ Tag
PK2
Cell Background
HEK 293
Agonist/Ligand
IGF-1
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist for 180 minutes at room temperature. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® Receptor Tyrosine Kinase Functional Assay

    PathHunter Receptor Tyrosine Kinase (RTK) Functional Assays are functional cell-based assays used for identification, screening or profiling of agonists, small molecule inhibitors and therapeutic biologics (peptides, proteins and various types of antibodies). Additionally, they have non-R&D applications as bioassays for QC testing and testing for anti-drug ...

    [User Manual] PathHunter® Receptor Tyrosine Kinase Functional Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2594.00
92-3105G AssayComplete™ Cell Culture Kit-105 Kit $339.00
92-5101FR AssayComplete™ Freezing Reagent F1 2 x 50 mL $288.00
93-0563R17A AssayComplete™ Cell Plating 17 Reagent 100 mL $288.00
92-4101TR AssayComplete™ Thawing Reagent T1 100 mL $236.00
92-0029 AssayComplete™ Hygromycin B 20 mL $175.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00