ChemiScreen™ CCR9 Chemokine Receptor Stable Cell Line

The CCR9 receptor is a GPCR expressed selectively in the thymus, lymph node, spleen, and the small bowel (SB). CCR9 binds to Thymus-expressed chemokine ligand (TECK / CCL25) (Zaballos et al. 1999, Yu et al., 2000, Carramolino et al., 2001), and is involved in directing the movement of mature T cells in the thymus. CCR9 also plays an important role in the pathogenesis of SB Crohn's disease (Papadakis et al, 2001). The CCR9 selective expression in the small bowel but not the colon indicates the the role of CCR9 in the specialized immune response in the SB. It was also shown that CCR9-mediated antiapoptosis could help in the study of cell survival mechanism and T cell malfunctioning. The cloned human CCR9-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR9 expression on the cell surface and contains high levels of the promiscuous Gα15 protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR9 and its ligands.

Catalog No. Size Price Quantity
HTS036C 2 Vials $9,669.00

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Application
Calcium Flux Assay
Family
Chemokine
Target
CCR9
Accession Number
NM_031200
Species
Human
Host Cell Type
Chem-1
Content
2 Vials of mycoplasma-free cells, 1 ml per vial
Storage
Cell vials are to be stored in the vapor phase of liquid N2.
  • [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines

    The ChemiSCREEN and ChemiBRITE Cell Lines are designed to enable the measurement of intracellular calcium levels when a GPCR of interest has been activated by a ligand. These cell lines are suitable for studying small molecules as well as biologics. The assay protocol has been optimized for use in either a 384-well or 96-well format.

    ChemiSCREEN and ChemiBRITE cells stably expres...

    [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines