Products / Cell Line / 97-1010C003
Contact a Specialist

KILR® U2OS-EGFR Cell Line

KILR cell lines are engineered to express an enhanced Prolabel (ePL) tagged housekeeping gene and may sometimes overexpress an untagged version of a receptor. Once the cells have been lysed the ePL-tagged protein is released into the media. Addition of enzyme acceptor (EA) will cause the complementation of the β-galactosidase enzyme fragments, EA and ePL. The resulting functional enzyme will hydrolyze its substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
97-1010C003 2 vials $14,989.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.
Cell Background
U2OS-EGFR
Cell Type
Osteosarcoma
Culture Mode
Adherent
Validation
Cetuximab
Agonist/Ligand
Cetuximab
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the indicated amount of time. Antibody was added and opsonized for the indicated time (below). Effector cells were added and the plate was incubated at 37°C/5% CO2 using the assay conditions described below. Target cell death was detected using the KILR detection reagent according to the recommended protocol. Additional reagents needed are noted on this document.

  • [User Manual] KILR® Cytotoxicity Assay−Adherent Target Cell Lines

    The KILR cytotoxicity cell line enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in Cancer Immunotherapy (iOnc) drug development including measuring target cell death through Antibody-Dependent Cell-mediated Cytotoxicity, ADCC, Complement-D...

    [User Manual] KILR® Cytotoxicity Assay−Adherent Target Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
97-0001M KILR® Detection Kit 1,000 dp (96-well) $1,479.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $389.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $334.00
93-0563R39A AssayComplete™ Cell Plating 39 Reagent 100 mL $334.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $249.00
92-0030 AssayComplete™ G418 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $114.00
92-0028 AssayComplete™ Puromycin 1 mL $97.00